In the last years, the number of organisms used for research has greatly expanded. Emerging models as well as models already currently much studied in labs provide a resource useful for answering scientific questions with the most adapted species, at lower costs and following the 3R rules.
In this context, the development of technologies for transgenesis and mutagenesis of imaging tools for phenotyping is crucial to cope with the awaited huge increase in functional approaches linked to the emergence of the revolutionary methods of precise genome editing in a rapidly groing number of models, including zebrafish and fruitfly.
The specific advantages of the two TEFOR’s models are the accessibility of their eggs, embryos, or larvae, which allow developing various studies. Both species share numerous physiological features and similar genetic tools.

Zebrafish (Danio rerio) constitutes an alternative compromise when greater relatedness to humans is warranted. Also, the advantages of zebrafish in preclinical toxicity screenings and research are numerous.

Fruitfly (Drosophila melanogaster) is one of the most tractable multicellular organisms for genetic analysis. The success of Drosophila melanogaster as a model organism was so far largely due to the power of forward genetic screens to identify the genes that are involved in a biological process. Recent emergence of genome editing methods will boost or complement these approaches.

Although tefor is currently centered on these two model species, it will be open to other model species all along its evolution, especially through the EFOR network (
TEFOR wishes to support research through the establishment of research calls via its own budget or in collaboration with other infrastructures, or other partners such as the fondation maladies rares. Furthermore, TEFOR aims at coordinating the creation of a novel european infrastructure, open to other model species, such as xenopus, chicken or quails...
TEFOR is composed of three labs performing transgenesis and mutagenesis, four phenotyping sites and four technological platforms in molecular biology, imaging and informatics. This unique consortium involving four academic partners forced us to settle well-defined protocols and tools to share results between partners and user of the platform. This will result in a growing pool of homogeneously acquired data enabling the correlation of genetic modifications, applied during the generation of the population, with the anatomic phenotypes observed in the imaged specimens.
The CRISPR Revolution
Guidance on the environmental risk assessment of genetically modified animals
PITCh-ing an Alternative Knock-in Strategy for TALENs and CRISPR/Cas9
EFOR - Réseau d'´Étude Fonctionnelle chez les ORganismes modèles
CRISPR Cas9: A novel approach to genetic engineering
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