Targeted insertions can now occur via very short micro-homologies.
Researchers based at Hiroshima University have worked out a new approach, the Precise Integration into Targeted Chromosome (PITCh) system. It relies on microhomology-mediated end joining (MMEJ) instead of homologous recombination (HR). PITCh, the researchers report, has been used with two different kinds of programmable nucleases, transcription activator-like effector nucleases (TALENs) and RNA-guided endonucleases, the latter of which features in the much-celebrated clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) system.

Find more in the links below:
The CRISPR Revolution
Guidance on the environmental risk assessment of genetically modified animals
PITCh-ing an Alternative Knock-in Strategy for TALENs and CRISPR/Cas9
EFOR - Réseau d'´Étude Fonctionnelle chez les ORganismes modèles
CRISPR Cas9: A novel approach to genetic engineering
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