Targeted insertions can now occur via very short micro-homologies.
Researchers based at Hiroshima University have worked out a new approach, the Precise Integration into Targeted Chromosome (PITCh) system. It relies on microhomology-mediated end joining (MMEJ) instead of homologous recombination (HR). PITCh, the researchers report, has been used with two different kinds of programmable nucleases, transcription activator-like effector nucleases (TALENs) and RNA-guided endonucleases, the latter of which features in the much-celebrated clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) system.
Find more in the links below:http://www.genengnews.com/gen-news-highlights/pitch-ing-an-alternative-knock-in-strategy-for-talens-and-crispr-cas9/81250665/http://www.nature.com/ncomms/2014/141120/ncomms6560/abs/ncomms6560.html